Description
KleverTest COVID-19 Express Kit was designed for fast
SARS-CoV-2 detection without preliminary RNA samples isolation and
purification. Clinical samples (in a saline or PBS transport media) can be used
directly for subsequent amplification. KleverTest COVID-19 Express Kit was
validated for the following Real-Time PCR instruments: Mx 3005PTM QPCR System
(Agilent), Rotor-Gene® 6000 (Corbett Research), Rotor-Gene® Q5/6 plex Platform
(QIAGEN), CFX96TM Systems (Bio-Rad). KleverTest COVID-19 Express Kit primers
and probes sets are designed to detect RNA from the SARS CoV-2 N genes
(HEX/Yellow channel) and RNase P internal control DNA (FAM/Green channel).
KleverTest COVID-19 Express assay primer and probe sequences are identical to
the FDA EUA authorized CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. An
additional in silico analysis assessing the impact of the recently emerged
SARS-CoV-2 variants on target detection by KleverTest COVID-19 showed that none
of the observed mutations would be expected to interfere with N genes
detection.
