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Identification and characterization of novel Cas protein orthologs
Caszyme offers identification and characterization of novel Cas orthologs. The workflow of this process:
-Bioinformatic analysis to select Cas protein of interest.
-dsDNA cleavage determination in cell-free and cell lysates.
-Determination of PAM sequence.
-Protein expression in E. coli and purification.
-Assessment of selected various properties: PAM and gRNA sequences, target sequence length, protein thermostability, nuclease activity.