Description
Instructions for Use
- Collect blood by venipuncture according to normal phlebotomy practice. TVTs are compatible with shielded needle devices from most major manufacturers.
- Fill tube completely. Blood will be aspirated up to the correct total volume and no further. This is important to avoid an incorrect TransFix to blood ratio that could affect results.
- Remove the TVT from the needle holder and immediately mix by gentle inversion 10 times to distribute the TransFix throughout the blood sample. Inadequate or delayed mixing may result in coagulation and inaccurate test results. Do not vortex.
- After collection, store/transport the blood filled TVT at 2 – 8°C for up to 14 days or for up to 3 days at 18 – 25°C.
- If refrigerated, incubate the TVT at room temperature (18 – 25°C) for 15 minutes prior to use.
- Mix the TransFix treated blood by rolling the TVT between the hands 10 times and by inverting 10 times. Heavier cells and blood components will sediment over the 14 day period, forming two distinct layers; this is normal.
- Remove and reinsert the cap by grasping with a simultaneous twisting and pulling action, not by a ‘thumb roll’ method.
- Perform immunophenotyping by flow cytometry in accordance with the manufacturer’s instructions. A ‘stain, lyse-no wash’ sample preparation method is recommended. Blood stabilized within TVTs should be analyzed within 6 hours before being returned to 2 – 8°C storage for future use, if necessary.
- When treated with Transfix the dilution factor must be accounted for when calculating absolute cell counts. Adjust the absolute cell count by multiplying the output by 1.2.
