Zena Max – COVID-19
Zena Max SARS-CoV-2 Real Time PCR Detection Kit detects the presence of COVID-19. The assay consists of a forward primer, a reverse primer and a probe labelled with the 5’ FAM™ and ROX reporter dye with a 3’ quencher. As the new target cDNA strand is synthesised, the tightly bound probe is cleaved by the 5’ to 3’ exonuclease activity of Taq polymerase which releases the fluorescent reporter from the quencher and substantially increases the fluorescent signal. The point at which the fluorescence becomes detectable above the background, the quantification cycle (Cq), is proportional to the amount of target present in the sample. The primers and probe sets target the RdRp gene and N Gene which had previously been used in the identification of the SARS coronavirus, however, there is no cross-reactivity with this or any other coronavirus sequenced thus far. .These assays are both incorporated into a ready-to-use PCR master mix which utilises hot start technology, thus minimising non-specific reactions and ensuring maximum sensitivity.
This in Vitro diagnostic kit provides qualitative detection.
The Zena Max COVID-19 PCR kit is sensitive down to a minimum of one copy per reaction under our validation methods and devices. The specificity of the AMD SARS-CoV-2 assay is up to 100% for Human SARS-CoV-2 Virus RNA.