KleverTest COVID-19 Express Kit

KleverTest COVID-19 Express Kit was designed for fast SARS-CoV-2 detection without preliminary RNA samples isolation and purification. Clinical samples (in a saline or PBS transport media) can be used directly for subsequent amplification. KleverTest COVID-19 Express Kit was validated for the following Real-Time PCR instruments: Mx 3005PTM QPCR System (Agilent), Rotor-Gene® 6000 (Corbett Research), Rotor-Gene® Q5/6 plex Platform (QIAGEN), CFX96TM Systems (Bio-Rad). KleverTest COVID-19 Express Kit primers and probes sets are designed to detect RNA from the SARS CoV-2 N genes (HEX/Yellow channel) and RNase P internal control DNA (FAM/Green channel). KleverTest COVID-19 Express assay primer and probe sequences are identical to the FDA EUA authorized CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. An additional in silico analysis assessing the impact of the recently emerged SARS-CoV-2 variants on target detection by KleverTest COVID-19 showed that none of the observed mutations would be expected to interfere with N genes detection.