Uracil-DNA Glycosylase

Uracil-DNA Glycosylase is an enzyme that catalyzes the hydrolysis of the N-glycosyl bond between uracil and the sugar residue in single- and double-stranded DNA. The enzyme possesses maximal activity at 37°C. Uracil-DNA Glycosylase is used in the preparation of PCR mixtures to prevent the appearance of false-positive results caused by contamination of the PCR reaction mixture with products of previously performed PCR (amplicons), which are destroyed before amplification. The enzyme is not active against RNA and short oligonucleotides. The enzyme is inactivated completely during the first cycle of DNA denaturation and does not interfere with the current reaction amplification products.